5YH1

Member of s1p family of ribosomal proteins PF0399 DHH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 63 2 2
Unit cell lengths	132.500, 132.500, 212.245
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.250 - 2.180
R-factor	0.187
Rwork	0.187
R-free	0.20200
RMSD bond length	0.006
RMSD bond angle	1.130
Phasing software	AutoSol
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.180
Number of reflections	56239
<I/σ(I)>	15
Completeness [%]	97.0
Redundancy	33.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	4% (V/V) PEG 400 100 mM Sodium acetate/ Acetic acid pH 5.5 2000 mM Lithium sulfate 100 mM Magnesium sulfate