5YGU

Crystal structure of Escherichia coli (strain K12) mRNA Decapping Complex RppH-DapF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 41 21 2
Unit cell lengths	86.418, 86.418, 173.093
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.985 - 2.298
R-factor	0.2077
Rwork	0.206
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4s2v 4ijz
RMSD bond length	0.009
RMSD bond angle	1.030
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.290
Number of reflections	30026
<I/σ(I)>	18.8
Completeness [%]	99.8
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	Potassium Sodium Tartrate, Sodium Iodine, Xylitol