5YGQ

Crystal Structure of Ferredoxin NADP+ Oxidoreductase from Rhodopseudomonas palustris

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-22
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	70.868, 81.227, 122.182
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.420 - 2.400
R-factor	0.22053
Rwork	0.218
R-free	0.26324
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ab1
RMSD bond length	0.018
RMSD bond angle	2.143
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.400	2.400
Rmerge	0.081	0.399
Number of reflections	27560
<I/σ(I)>	8.5	2.3
Completeness [%]	98.3	88.1
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 4000, ammonium iodide, Tris-HCl pH8.5