5YGP
Human TNFRSF25 death domain mutant-D412E
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 110 |
| Detector technology | PIXEL |
| Collection date | 2016-06-26 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.978500 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.620, 155.020, 74.030 |
| Unit cell angles | 90.00, 90.18, 90.00 |
Refinement procedure
| Resolution | 46.990 - 2.090 |
| Rwork | 0.218 |
| R-free | 0.24650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5h7q |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1.12-2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.990 | 2.167 |
| High resolution limit [Å] | 2.090 | 2.092 |
| Rmerge | 0.557 | |
| Number of reflections | 126843 | |
| <I/σ(I)> | 10.57 | |
| Completeness [%] | 98.0 | 91.22 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 2 M ammonium sulfate, sodium citrate 5.6, 10% glycerol |
