5YGG

Crystal structure of Fructokinase Double-Mutant (T261C-H108C) from Vibrio cholerae O395 in fructose, ADP and potassium ion bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-16
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 2 21 21
Unit cell lengths	41.760, 64.330, 116.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.060 - 1.671
R-factor	0.1647
Rwork	0.163
R-free	0.19470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ey7
RMSD bond length	0.012
RMSD bond angle	1.115
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.920
High resolution limit [Å]	1.670
Number of reflections	36538
<I/σ(I)>	9.6
Completeness [%]	97.5
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	PEG 6000, calcium chloride, ADP, Fructose, Potassium Chloride