5YGD

Crystal structure of Drosophila melanogaster Papi extended Tudor domain (D287A) in complex with Piwi N-terminal R10me2s peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 41
Unit cell lengths	72.472, 72.472, 50.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.403 - 1.551
R-factor	0.2047
Rwork	0.203
R-free	0.22970
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3fdr
RMSD bond length	0.007
RMSD bond angle	1.104
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.610
High resolution limit [Å]	1.550	1.550
Rmerge	0.060	0.430
Number of reflections	37809
<I/σ(I)>	34.1	2.3
Completeness [%]	99.9	99.8
Redundancy	10.8	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	290	0.2 M Sodium malonate pH7.0, 20% (w/v) PEG3350