5YF6
Crystals structure of Classical swine fever virus NS5B (residues 1-682)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 161.352, 161.352, 55.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.987 - 2.100 |
| R-factor | 0.1819 |
| Rwork | 0.180 |
| R-free | 0.22700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cjq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.800 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 4.520 | 2.100 |
| Rmerge | 0.066 | 0.037 | 0.459 |
| Rmeas | 0.070 | 0.040 | 0.483 |
| Rpim | 0.022 | 0.013 | 0.150 |
| Total number of observations | 448970 | ||
| Number of reflections | 43794 | 4675 | 4297 |
| <I/σ(I)> | 11.9 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 10.3 | 9.5 | 10.3 |
| CC(1/2) | 0.999 | 0.955 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8 | 289 | Polypropylene glycol 400 |
