5YEJ
Crystal structure of BioQ with its naturel double-stranded DNA operator
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-31 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 60.687, 212.468, 88.126 |
| Unit cell angles | 90.00, 99.43, 90.00 |
Refinement procedure
| Resolution | 37.676 - 2.698 |
| R-factor | 0.2141 |
| Rwork | 0.212 |
| R-free | 0.26530 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.074 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 45.000 |
| High resolution limit [Å] | 2.690 |
| Number of reflections | 29957 |
| <I/σ(I)> | 7.7 |
| Completeness [%] | 98.0 |
| Redundancy | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | PEG 8000, magnesium acetate, 1-propanol, MOPS, HEPES |
