5YEE

Crystal structure of LokiProfilin1/Rabbit Actin Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-20
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	43.312, 126.209, 53.862
Unit cell angles	90.00, 97.89, 90.00

Refinement procedure

Resolution	63.100 - 1.810
R-factor	0.19422
Rwork	0.191
R-free	0.24864
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hbt 5yed
RMSD bond length	0.018
RMSD bond angle	1.943
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.100	1.840
High resolution limit [Å]	1.810	1.810
Rmerge		0.692
Number of reflections	49614
<I/σ(I)>	11.9
Completeness [%]	99.5	78.5
Redundancy	5.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	100 mM Hepes, pH 7.0, 20 % w/v Polyethylene glycol 6,000, 200 mM NaCl, 10 mM ATP disodium salt