5YDC
Crystal structure of mercury soaked C-terminal domain of Rv1828 from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0080 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.355, 31.511, 77.901 |
| Unit cell angles | 90.00, 98.21, 90.00 |
Refinement procedure
| Resolution | 44.890 - 1.910 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.26000 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.547 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.905 | 1.910 |
| Rmerge | 0.100 | 1.280 |
| Number of reflections | 16754 | |
| <I/σ(I)> | 20 | 3.1 |
| Completeness [%] | 97.3 | 95.4 |
| Redundancy | 7.9 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 2.2 M Ammonium sulfate, 0.1 M Bis-Tris (pH 6.5) |
