5YDA

The crystal structure of the Acyl Transferase domain of SpnD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 43 2 2
Unit cell lengths	109.780, 109.780, 142.573
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.095 - 2.353
R-factor	0.1962
Rwork	0.195
R-free	0.21510
RMSD bond length	0.008
RMSD bond angle	1.059
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX (1.10.1-2155)
Refinement software	PHENIX (1.10.1-2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.390
High resolution limit [Å]	2.350	2.350
Rmerge	0.078	0.692
Number of reflections	36828
<I/σ(I)>	49
Completeness [%]	100.0
Redundancy	25.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	1.5M Lithium sulfate monohydrate, 0.1M Bis-Tris propane pH 7.0