5YCA

Crystal structure of inner membrane protein Bqt4 in complex with LEM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	43.868, 53.128, 109.566
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.392 - 1.570
R-factor	0.1906
Rwork	0.189
R-free	0.21360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ybx 5yc2
RMSD bond length	0.006
RMSD bond angle	1.167
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.392	1.600
High resolution limit [Å]	1.570	1.570
Rmerge		0.264
Rmeas		0.276
Rpim		0.079
Number of reflections	36534
<I/σ(I)>	3.7
Completeness [%]	99.9	98.6
Redundancy	3	11.6
CC(1/2)		0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	289	1600 mM Sodium citrate tribasic