5YBZ

High resolution structure of complement C1q-like protein 3 C1q domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BSRF BEAMLINE 3W1A
Synchrotron site	BSRF
Beamline	3W1A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-10-12
Detector	MAR scanner 345 mm plate
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	54.027, 79.213, 87.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.074 - 1.711
R-factor	0.1537
Rwork	0.152
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.023
Phasing software	HKL-3000
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.760
High resolution limit [Å]	1.711	1.711
Rmerge	0.076	0.457
Number of reflections	40768	1976
<I/σ(I)>	26.5	2.9
Completeness [%]	99.8	98.1
Redundancy	6.5	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	ammonium sulfate, sodium citrate, manganese chloride