5YBH

Structural of the highly conserved ATPase from type III secretion system of bacterial pathogens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97876
Spacegroup name	P 32 2 1
Unit cell lengths	105.385, 105.385, 146.386
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.495 - 2.500
R-factor	0.1946
Rwork	0.192
R-free	0.24800
RMSD bond length	0.009
RMSD bond angle	1.135
Data reduction software	XDS
Data scaling software	XDS
Phasing software	SHARP
Refinement software	PHENIX (1.9_1690)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.105	0.590
Number of reflections	32716
<I/σ(I)>	25.38	5.6
Completeness [%]	97.9	96.6
Redundancy	16.4	15.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.6	296	0.1M Bicine pH8.6;1.2M Ammonium sulphate