5YBE

Structure of KANK1/KIF21A complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97538
Spacegroup name	P 21 21 21
Unit cell lengths	35.513, 51.958, 136.984
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.395 - 2.111
R-factor	0.1836
Rwork	0.180
R-free	0.24560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hbd
RMSD bond length	0.013
RMSD bond angle	1.215
Data scaling software	SCALEPACK
Phasing software	PHENIX (1.10_2155)
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.140
High resolution limit [Å]	2.100	5.700	2.100
Rmerge	0.104	0.060	0.422
Rmeas	0.118	0.068	0.517
Rpim	0.054	0.031	0.292
Number of reflections	14123	857	484
<I/σ(I)>	5.7
Completeness [%]	92.2	97.9	65.2
Redundancy	4.3	4.7	2.5
CC(1/2)		0.996	0.761

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	289	0.1M sodium formate pH8.0, 14% PEG3,350