5YBC

X-ray structure of native ETS-domain domain of Ergp55

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2013-04-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97625
Spacegroup name	P 41 21 2
Unit cell lengths	64.565, 64.565, 128.305
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.283 - 2.500
R-factor	0.2243
Rwork	0.222
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.368
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.068	0.032
Number of reflections	9971	580	487
<I/σ(I)>	11.8
Completeness [%]	99.6	97.3	99.4
Redundancy	27.5	23.4	25.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	277	20%(w/v) PEG400, 130mM NaCl, 60mM MgCl2, 100mM sodium citrate pH 5.6