5YB8

L-Amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 - L-arginine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	98.200, 133.000, 101.500
Unit cell angles	90.00, 112.00, 90.00

Refinement procedure

Resolution	94.110 - 2.300
R-factor	0.2208
Rwork	0.220
R-free	0.27290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3we0
RMSD bond length	0.015
RMSD bond angle	1.726
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.110	94.110	2.420
High resolution limit [Å]	2.300	15.000	2.300
Rmerge	0.081	0.040	0.472
Rmeas	0.094	0.047	0.550
Total number of observations	404779
Number of reflections	106954	374	15105
<I/σ(I)>	11.98	28.14	3.24
Completeness [%]	99.7	89.9	99.8
Redundancy	3.785	3.316	3.805
CC(1/2)	0.997	0.998	0.885

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% PEG 3350, 0.15M DL-Malic acid