5YB7

L-Amino acid oxidase/monooxygenase from Pseudomonas sp. AIU 813 - L-ornithine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-10-29
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	94.600, 132.900, 101.900
Unit cell angles	90.00, 111.50, 90.00

Refinement procedure

Resolution	94.810 - 2.000
R-factor	0.1985
Rwork	0.198
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3we0
RMSD bond length	0.017
RMSD bond angle	1.769
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	94.810	94.810	2.150
High resolution limit [Å]	2.000	15.000	2.000
Rmerge	0.054	0.021	0.407
Rmeas	0.063	0.025	0.474
Number of reflections	156339	345	30060
<I/σ(I)>	16.01	45.36	3.7
Completeness [%]	98.9	84.8	98
Redundancy	3.779	3.388	3.746
CC(1/2)	0.999	0.999	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	20% PEG 3350, 0.15 M DL-Malic acid