5YB3

Crystal structure of HP23L/N36

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9796
Spacegroup name	P 43 21 2
Unit cell lengths	51.148, 51.148, 168.736
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.170 - 2.043
R-factor	0.1897
Rwork	0.188
R-free	0.23100
RMSD bond length	0.007
RMSD bond angle	0.772
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	36.170
High resolution limit [Å]	2.040
Number of reflections	15003
<I/σ(I)>	26.5
Completeness [%]	99.4
Redundancy	18.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.05M Sodium cacodylate trihydrate pH 6.0,30%(v)/v (+/-)-2-Methyl-2,4-pentanedio