5YB1
Structure and function of human serum albumin-metal agent complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-12-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.492, 95.653, 96.642 |
| Unit cell angles | 104.67, 101.43, 89.97 |
Refinement procedure
| Resolution | 33.000 - 2.616 |
| R-factor | 0.2216 |
| Rwork | 0.219 |
| R-free | 0.27830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bj5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.810 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.680 |
| High resolution limit [Å] | 2.616 | 2.616 |
| Rmerge | 0.080 | 0.298 |
| Number of reflections | 38702 | |
| <I/σ(I)> | 4.1 | 4.1 |
| Completeness [%] | 98.0 | 98.2 |
| Redundancy | 3.3 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG3350, MPD, DMSO |
