5YAX

Crystal structure of a human neutralizing antibody bound to a HBV preS1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 21 21 2
Unit cell lengths	142.670, 55.662, 67.696
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.923 - 2.500
R-factor	0.2448
Rwork	0.242
R-free	0.28790
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.540
High resolution limit [Å]	2.500	2.500
Rmerge	0.144	0.321
Number of reflections	19392	886
<I/σ(I)>	17.7	6.2
Completeness [%]	96.0	94.9
Redundancy	5.8	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium acetate