5YAQ
Crystal structure of scyllo-inositol dehydrogenase with L-glucose dehydrogenase activity complexed with scyllo-inosose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-02-19 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 86.550, 121.243, 140.395 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.000 - 1.990 |
| R-factor | 0.18073 |
| Rwork | 0.180 |
| R-free | 0.19352 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5yab |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.539 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.074 | 0.381 |
| Number of reflections | 100761 | |
| <I/σ(I)> | 22.9 | 3.6 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 4.1 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M sodium acetate pH 4.8 - 5.4, and 12 - 35% PEG3350 |
