5YAB
Crystal structure of scyllo-inositol dehydrogenase with L-glucose dehydrogenase activity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.974, 127.453, 137.535 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.300 - 1.750 |
R-factor | 0.17734 |
Rwork | 0.177 |
R-free | 0.19184 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.356 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.055 | 0.247 |
Number of reflections | 160030 | |
<I/σ(I)> | 24.7 | 4.9 |
Completeness [%] | 99.3 | 94.4 |
Redundancy | 3.7 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 12 - 35% PEG3350, 0.1 M sodium acetate, pH 4.8 - 5.4 |