5Y9D
Crystal structure of acyl-coA oxidase1 from Yarrowia lipolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-18 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | I 4 |
| Unit cell lengths | 167.884, 167.884, 95.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.950 - 2.500 |
| R-factor | 0.1937 |
| Rwork | 0.183 |
| R-free | 0.25340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | pdbid 1W07 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.497 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 6.780 | 2.500 |
| Rmerge | 0.127 | 0.095 | 0.316 |
| Rmeas | 0.142 | 0.106 | 0.366 |
| Rpim | 0.062 | 0.046 | 0.181 |
| Total number of observations | 207931 | ||
| Number of reflections | 45463 | 2326 | 2229 |
| <I/σ(I)> | 14.9 | ||
| Completeness [%] | 99.1 | 97.9 | 98.3 |
| Redundancy | 4.6 | 5.1 | 3.7 |
| CC(1/2) | 0.986 | 0.785 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | PEG 3350, NaCit, Tacsimate |
