5Y9D

Crystal structure of acyl-coA oxidase1 from Yarrowia lipolytica

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2015-05-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	I 4
Unit cell lengths	167.884, 167.884, 95.313
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.950 - 2.500
R-factor	0.1937
Rwork	0.183
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	pdbid 1W07
RMSD bond length	0.011
RMSD bond angle	1.497
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.127	0.095	0.316
Rmeas	0.142	0.106	0.366
Rpim	0.062	0.046	0.181
Total number of observations	207931
Number of reflections	45463	2326	2229
<I/σ(I)>	14.9
Completeness [%]	99.1	97.9	98.3
Redundancy	4.6	5.1	3.7
CC(1/2)		0.986	0.785

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	PEG 3350, NaCit, Tacsimate