5Y8R

ZsYellow at pH 3.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-26
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9795
Spacegroup name	I 41 2 2
Unit cell lengths	111.178, 111.178, 103.751
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.160 - 2.300
R-factor	0.208
Rwork	0.206
R-free	0.25750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ogr
RMSD bond length	0.016
RMSD bond angle	1.981
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.300
Number of reflections	13860
<I/σ(I)>	39.421
Completeness [%]	94.3
Redundancy	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295.5	Na-citrate, NaCl