5Y8F

Crystal Structure of a prokaryotic SEFIR domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	173
Detector technology	IMAGE PLATE
Collection date	2013-01-25
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	37.860, 68.017, 54.941
Unit cell angles	90.00, 97.51, 90.00

Refinement procedure

Resolution	29.177 - 2.000
R-factor	0.1875
Rwork	0.186
R-free	0.22190
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.982
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.180
High resolution limit [Å]	2.000
Number of reflections	271348
<I/σ(I)>	12.7
Completeness [%]	94.9
Redundancy	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M NaCl, 0.1 M imidozale pH 8.0, 30% PEG 8000