5Y8E
Crystal Structure of a prokaryotic SEFIR domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2012-12-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.739, 61.673, 39.272 |
| Unit cell angles | 90.00, 115.90, 90.00 |
Refinement procedure
| Resolution | 35.327 - 1.804 |
| R-factor | 0.1683 |
| Rwork | 0.167 |
| R-free | 0.19630 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.963 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXCD |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.330 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 108121 |
| <I/σ(I)> | 11.2 |
| Completeness [%] | 99.4 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 289 | 0.2 M NaCl, 0.1 M imidozale pH 8.0, 30% PEG 8000 |
