5Y84
Hapalindole U and DMSPP Bound AmbP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-06-06 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.311, 54.810, 77.087 |
| Unit cell angles | 105.42, 106.24, 98.68 |
Refinement procedure
| Resolution | 26.428 - 2.000 |
| R-factor | 0.2136 |
| Rwork | 0.211 |
| R-free | 0.25850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y4g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.894 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.600 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.465 | |
| Number of reflections | 44715 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 97.4 | 96.6 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MES, PEG 8000, magnesium chloride |
