5Y7D

Crystal structure of human Endothelial-overexpressed LPS associated factor 1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	193
Detector technology	CCD
Collection date	2014-10-22
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	49.765, 49.765, 175.709
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.933 - 1.710
R-factor	0.2017
Rwork	0.199
R-free	0.24690
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.703
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.890
High resolution limit [Å]	1.709
Number of reflections	24918
<I/σ(I)>	53.11
Completeness [%]	99.8
Redundancy	29.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.1M HEPES, 4.3M NaCl