5Y7C
Hapalindole A and DMSPP Bound AmbP3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 46.465, 54.724, 77.991 |
| Unit cell angles | 105.23, 106.60, 98.51 |
Refinement procedure
| Resolution | 37.837 - 2.003 |
| R-factor | 0.1977 |
| Rwork | 0.195 |
| R-free | 0.24270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y4g |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.841 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.476 | |
| Number of reflections | 45052 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 97.2 | 95.4 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | MES, PEG 8000, magnesium chloride |
