5Y6O

Crystal structure of DAXX N-terminal four-helix bundle domain (4HB) in complex with ATRX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-10
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 31 2 1
Unit cell lengths	78.666, 78.666, 503.215
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	83.869 - 3.100
R-factor	0.2274
Rwork	0.224
R-free	0.29720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2kzs
RMSD bond length	0.010
RMSD bond angle	1.434
Data scaling software	pointless
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	167.740	3.270
High resolution limit [Å]	3.100	3.100
Rmerge	0.091	1.344
Rmeas	0.097	1.449
Rpim	0.033	0.527
Number of reflections	34423	4917
<I/σ(I)>	14.4	1.1
Completeness [%]	99.9	99.9
Redundancy	8	6.9
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.8	293	The DAXX 4HB_ATRX DBM fusion protein at 26 mg/ml was crystallized under conditions of 0.1M Sodium Cacodylate, pH 6.8, 1.2 M Ammonium Sulfate and 3% 1, 5- Diaminopentane Dihydrochloride, using sitting-drop vapor-diffusion method at 293K. In this process, o.5 uL of protein was mixed with 0.5 uL of mother liquor. All the crystals were soaked in a cryoprotectant made from mother liquor supplemented with 25% glycerol before flash freezing in liquid nitrogen.