5Y6M
Zika virus helicase in complex with ADP-AlF3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97778 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 53.638, 69.152, 57.819 |
| Unit cell angles | 90.00, 94.14, 90.00 |
Refinement procedure
| Resolution | 44.289 - 2.002 |
| R-factor | 0.179 |
| Rwork | 0.177 |
| R-free | 0.22340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5gjc |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.271 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2148: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | |
| Number of reflections | 26053 | 1327 |
| <I/σ(I)> | 7.9 | 1 |
| Completeness [%] | 91.7 | 93.9 |
| Redundancy | 2.5 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 291.15 | 0.1M BIS-TRIS (pH 5.5), 25% w/v polyethylene glycol 3350 |
