5Y5V
Crystal structure of a novel Pyrethroid Hydrolase PytH (S78A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-01-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 168.200, 168.200, 123.626 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.823 - 2.980 |
| R-factor | 0.2194 |
| Rwork | 0.216 |
| R-free | 0.29150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.199 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2247: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.140 |
| High resolution limit [Å] | 2.980 | 2.980 |
| Number of reflections | 36638 | 5259 |
| <I/σ(I)> | 7.1 | 2.2 |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 9.8 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | Ammonium Sulfate |
