5Y5R
Crystal structure of a novel Pyrethroid Hydrolase PytH with BIF
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-03-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9793 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 168.857, 168.857, 123.864 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.913 - 1.899 |
R-factor | 0.2756 |
Rwork | 0.273 |
R-free | 0.32200 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.461 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((dev_2247: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.010 |
High resolution limit [Å] | 1.899 | 1.900 |
Number of reflections | 139606 | 41697 |
<I/σ(I)> | 7.91 | 1.18 |
Completeness [%] | 99.3 | 96.1 |
Redundancy | 14.9 | 14.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 293 | Ammonium Sulfate |