5Y5C
Crystal structure of dha bind to bovine beta-lactoglobulin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17B1 |
Synchrotron site | SSRF |
Beamline | BL17B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-14 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97775 |
Spacegroup name | P 32 |
Unit cell lengths | 52.490, 52.490, 108.390 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.460 - 2.610 |
R-factor | 0.20207 |
Rwork | 0.200 |
R-free | 0.24389 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gj5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.651 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | BALBES |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.500 |
Rmerge | 0.085 |
Number of reflections | 11464 |
<I/σ(I)> | 24 |
Completeness [%] | 98.4 |
Redundancy | 9.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 289 | 1.34M trisodium citrate, 0.1M Tris |