5Y59
Crystal structure of Ku80 and Sir4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97851 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.472, 79.472, 82.926 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.642 - 2.402 |
| R-factor | 0.2037 |
| Rwork | 0.201 |
| R-free | 0.24790 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.306 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 5.170 | 2.400 |
| Rmerge | 0.071 | 0.031 | 0.888 |
| Rmeas | 0.073 | 0.032 | 0.927 |
| Rpim | 0.017 | 0.007 | 0.253 |
| Total number of observations | 219073 | ||
| Number of reflections | 12169 | 1313 | 1156 |
| <I/σ(I)> | 6.2 | ||
| Completeness [%] | 99.6 | 100 | 96.2 |
| Redundancy | 18 | 17.3 | 11.4 |
| CC(1/2) | 1.000 | 0.833 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 7.5 | 277 | 0.1 M HEPES, 2.0 M ammonium sulfate |
| 1 | EVAPORATION | 7.5 | 277 | 0.1 M HEPES, 2.0 M ammonium sulfate |
