5Y57

Crystal structure of a Novel Pyrethroid Hydrolase from Sphingobium faniae JZ-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 42 21 2
Unit cell lengths	168.453, 168.453, 123.593
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.852 - 1.961
R-factor	0.2577
Rwork	0.255
R-free	0.30870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.127
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((dev_2247: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.070
High resolution limit [Å]	1.960	1.960
Number of reflections	125394	16979
<I/σ(I)>	9.2
Completeness [%]	98.9	92.9
Redundancy	11.7	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	Ammonium sulfate