5Y4L
PRRSV nsp4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-04-05 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.9790 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.681, 86.117, 142.955 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.544 - 2.198 |
R-factor | 0.2145 |
Rwork | 0.213 |
R-free | 0.25220 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.169 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.190 |
Number of reflections | 48853 |
<I/σ(I)> | 11.14 |
Completeness [%] | 92.4 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M Succinic acid pH 7.0, 15% w/v Polyethylene glycol 3350 |