5Y4K
Crystal structure of DddY mutant Y260A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-16 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.841, 72.646, 89.547 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.420 - 2.000 |
R-factor | 0.1461 |
Rwork | 0.144 |
R-free | 0.18780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xkx |
RMSD bond length | 0.007 |
RMSD bond angle | 0.794 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.167 | |
Number of reflections | 27489 | |
<I/σ(I)> | 41.6 | 18 |
Completeness [%] | 100.0 | 100 |
Redundancy | 24.9 | 23.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl (pH 8.5) and 25% PEG 3350 |