5Y42
Native-crystal structure of three chain non-toxic type II ribosome inactivating protein purified from the seeds of Trichosanthes anguina
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-09-23 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 64 |
| Unit cell lengths | 171.226, 171.226, 75.850 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 85.010 - 2.900 |
| R-factor | 0.20476 |
| Rwork | 0.202 |
| R-free | 0.25538 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hr6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.691 |
| Data reduction software | iMOSFLM (7.2) |
| Data scaling software | SCALA (3.3.22) |
| Phasing software | PHASER (2.7.15) |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.610 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.198 | |
| Number of reflections | 28365 | 4115 |
| <I/σ(I)> | 12.6 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.2 | 11.8 |
| CC(1/2) | 0.996 | 0.648 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2M lithium sulfate monohydrate, 0.1M Tris-Hcl, pH8.5, 30% PEG4000 |
