5Y40
Structure of the periplasmic domain of the MotB L119P mutant from Salmonella (crystal form 2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-16 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 150.950, 150.950, 91.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.077 - 2.800 |
| R-factor | 0.2183 |
| Rwork | 0.216 |
| R-free | 0.25010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zvy |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.751 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 91.400 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.106 | 0.624 |
| Number of reflections | 26584 | 3799 |
| <I/σ(I)> | 15.8 | 4.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 12.4 | 12.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 13% PEG 3350, 0.1M imidazole-HCl, 0.1M Arg-HCl, 5% MPD |
