5Y3B

Crystal structure of mouse Ccd1 DIX domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	72.854, 75.660, 125.595
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.088 - 3.000
R-factor	0.2496
Rwork	0.247
R-free	0.28940
Structure solution method	MAD
RMSD bond length	0.003
RMSD bond angle	0.798
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	autoSHARP
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.000
Number of reflections	14312
<I/σ(I)>	16.3
Completeness [%]	99.0
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293	0.1 M Na HEPES pH 7.8, 15%(v/v) ethylene glycol, 3%(v/v) glycerol, 4%(v/v) 1,3-propanediol