5Y3B
Crystal structure of mouse Ccd1 DIX domain
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-02-20 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 72.854, 75.660, 125.595 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.088 - 3.000 |
R-factor | 0.2496 |
Rwork | 0.247 |
R-free | 0.28940 |
Structure solution method | MAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.798 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | autoSHARP |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.000 |
Number of reflections | 14312 |
<I/σ(I)> | 16.3 |
Completeness [%] | 99.0 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 0.1 M Na HEPES pH 7.8, 15%(v/v) ethylene glycol, 3%(v/v) glycerol, 4%(v/v) 1,3-propanediol |