5Y2W
Structure of Synechocystis PCC6803 CcmR regulatory domain in complex with 2-PG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97861 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 58.965, 58.965, 123.958 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.260 - 2.200 |
| R-factor | 0.20308 |
| Rwork | 0.201 |
| R-free | 0.25476 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5y2v |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.743 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 13083 | 1289 |
| <I/σ(I)> | 11.4 | 3.3 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 4.8 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2 M MgCl2, 0.1 M HEPES, pH 7.5, 30% (v/v) Polyethylene glycol 400, 5 mM 2-PG |
