5Y2V
Strcutrue of the full-length CcmR complexed with 2-OG from Synechocystis PCC6803
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97906 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 90.766, 109.160, 178.226 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.19881 |
| Rwork | 0.197 |
| R-free | 0.23374 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.758 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.630 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 52491 | 2646 |
| <I/σ(I)> | 36.1 | 3.3 |
| Completeness [%] | 95.3 | 97.5 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2 M sodium citrate tribasicdihydrate, 0.1 M HEPES, pH 7.5, 20% (v/v) isopropanol |
