5Y2H
Crystal structure of the oligomerization domain of NSP4 from the rotavirus strain MF66
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-08-05 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 87.440, 87.440, 60.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 75.730 - 2.600 |
| R-factor | 0.2362 |
| Rwork | 0.235 |
| R-free | 0.26270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.229 |
| Data reduction software | Aimless |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1-2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 75.730 |
| High resolution limit [Å] | 2.600 |
| Rmerge | 0.150 |
| Rpim | 0.073 |
| Number of reflections | 8464 |
| <I/σ(I)> | 8.9 |
| Completeness [%] | 99.8 |
| Redundancy | 5.9 |
| CC(1/2) | 0.993 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 299 | 0.1M Citric acid, pH 3.5, 3.0M NaCl |
