5Y1E
monomeric L-threonine 3-dehydrogenase from metagenome database (L-Ser and NAD+ bound form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-06 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.594, 50.857, 55.252 |
| Unit cell angles | 90.00, 105.60, 90.00 |
Refinement procedure
| Resolution | 54.220 - 1.900 |
| R-factor | 0.16022 |
| Rwork | 0.158 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wmx |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.489 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.200 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 23337 | |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 3350, 0.2M Sodium fluoride |
