5Y1D
Monomeric L-threonine 3-dehydrogenase from metagenome database (apo form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.10 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 56.386, 49.319, 113.602 |
Unit cell angles | 90.00, 89.90, 90.00 |
Refinement procedure
Resolution | 113.600 - 1.900 |
R-factor | 0.19225 |
Rwork | 0.190 |
R-free | 0.23328 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3wmx |
RMSD bond length | 0.010 |
RMSD bond angle | 1.335 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 113.600 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 46791 | |
<I/σ(I)> | 16.2 | |
Completeness [%] | 95.7 | 96.5 |
Redundancy | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 20% (w/v) PEG 3350, 0.2M Potassium formate |