5XZX

Crystal structure of importin-alpha3 bound to the nuclear localization signal of Ran-binding protein 3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2011-02-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98
Spacegroup name	P 1 21 1
Unit cell lengths	47.970, 60.220, 85.020
Unit cell angles	90.00, 100.54, 90.00

Refinement procedure

Resolution	37.130 - 3.000
R-factor	0.2292
Rwork	0.227
R-free	0.28070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4uae
RMSD bond length	0.002
RMSD bond angle	0.502
Data scaling software	Aimless (0.5.32)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.860	48.860	3.180
High resolution limit [Å]	3.000	9.000	3.000
Rmerge	0.053	0.022	0.268
Rmeas	0.064	0.026	0.318
Rpim	0.034	0.014	0.168
Total number of observations	33080
Number of reflections	9597
<I/σ(I)>	12
Completeness [%]	99.1	94.5	99.6
Redundancy	3.4	3.3	3.5
CC(1/2)	0.998	0.999	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	PEG 8000