5XZU
Crystal structure of GH10 xylanase from Bispora. sp MEY-1 with xylobiose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00919 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.274, 83.136, 65.387 |
| Unit cell angles | 90.00, 94.25, 90.00 |
Refinement procedure
| Resolution | 25.000 - 1.700 |
| R-factor | 0.12691 |
| Rwork | 0.124 |
| R-free | 0.18323 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.489 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 1.700 |
| Number of reflections | 79182 |
| <I/σ(I)> | 5.96 |
| Completeness [%] | 99.9 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298.15 | 19% PEG 3350, 0.4 M magnesium chloride, 0.1 M BIS-TRIS HYDROCHLORIDE pH 6.5 |
