5XYA
Crystal structure of a serine protease from Streptococcus species
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-10-16 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 190.530, 190.530, 248.668 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.899 - 3.000 |
| R-factor | 0.2245 |
| Rwork | 0.223 |
| R-free | 0.27830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xxz |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.423 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (dev_2733) |
| Refinement software | PHENIX ((dev_2733: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.976 |
| High resolution limit [Å] | 3.000 | 2.900 |
| Rmerge | 0.170 | 0.910 |
| Number of reflections | 104019 | 3471 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 98.9 | |
| Redundancy | 24.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.2 M MgCl hexahydrate, 0.1 M Tris, pH 8.65, and 28% PEG3500 |
